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Keratinocyte Growth Factor Receptor Restores 5-Fluorouracil and Tamoxifen Efficacy on Resp

Silencing of Keratinocyte Growth Factor Receptor Restores 5-Fluorouracil and Tamoxifen Efficacy on Responsive Cancer Cells.Sabrina Rotolo 1, Simona Ceccarelli 1, Ferdinando Romano 1, Luigi Frati 1,2, Cinzia Marchese 1, Antonio Angeloni 1* 1 Dipartimento di Medicina Sperimentale, Universita` Sapienza di Roma, Roma, Italy, 2 Istituto Neurologico Mediterraneo ‘‘Neuromed, Pozzilli, ItalyAbstract : BackgroundKeratinocyte growth factor receptor (KGFR) is a splice variant of the FGFR2 gene expressed in epithelial cells. Activation of KGFR is a key factor in the regulation of physiological processes in epithelial cells such as proliferation, differentiation and wound healing. Alterations of KGFR signaling have been linked to the pathogenesis of different epithelial tumors. It has been also hypothesized that its specific ligand, KGF, might contribute to the development of resistance to 5-fluorouracil (5-FU) in epithelial cancers and tamoxifen in estrogen-positive breast cancers.Abstract : Methodology/Principal FindingsSmall interfering RNA was transfected into a human keratinocyte cell line (HaCaT), a breast cancer derived cell line (MCF-7) and a keratinocyte primary culture (KCs) to induce selective downregulation of KGFR expression. A strong and highly specific reduction of KGFR expression was observed at both RNA (reduction = 75.7%, P = 0.009) and protein level. KGFR silenced cells showed a reduced responsiveness to KGF treatment as assessed by measuring proliferation rate (14.2% versus 39.0% of the control cells, P

Nuovi fondi per la lotta alla contraffazione

Sicurezza, De Corato: “Identità sconosciute pericolo per Milano, 250 mila euro per potenziare laboratorio falsi e fotosegnalamento clandestini. fondi anche per raddoppiare lo street control”. Grazie a contributi regionali. Nel 2010 la Polizia Locale ha scoperto 1.226 documenti contraffatti e rilevato le impronte digitali a 1.786 soggetti. Da 20 a 40 le autovetture per il sistema ’a strascico’.

Taxi, al via le misure di sicurezza

Il vicesindaco Riccardo De Corato ha incontrato una delegazione di tassisti: “Da dicembre il collegamento con la control room della Polizia Locale e il bando per l’acquisto dei sistemi antirapina“

Master Plan, Sicurezza. Telecamere intelligenti

De Corato: "Telecamere ‘intelligenti, una nuova control room, mappa del rischio in tempo reale. Ma anche più presenza sulle strade per contrastare degrado e criminalità”

Prostituzione: Speriamo che Consiglio Ministri licenzi ddl Carfagna

Ogni notte Control room fotografa la Milano a "luci rosse”. Dai rumeni di piazza Trento ai viados di Lagosta alle prostitute di viale Abruzzi. Ma senza revisione normativa non ci sono mezzi per contrastare fenomeno

La ESA cambia por primera vez el sistema de control de un telescopio espacial en órbita

FOOD AND INDUSTRIAL MICROBIOLOGY

Food spoilage, food infections and intoxications caused by microorganisms and methods for their detection Dr. Neeraj Dilbaghi. Reader, Department of Bio & Nano Technology. Guru Jambheshwar University of Science and Technology Hisar- 125001 and Dr (Mrs.) S. Sharma. Professor, Department of Microbiology, CCS Haryana Agricultural University, Hisar- 125001 (Revised 25-Sep-2007) IntroductionFood Spoilage and General Principles Underlying Spoilage of Food Intrinsic Parameters Extrinsic Parameters Microbial Spoilage of Foods Spoilage of fresh and ready-to-eat meat products Spoilage of milk and milk products Spoilage of fruits and vegetables Spoilage of canned foods Major Food Borne Infections/ Intoxications Caused by Bacteria General Control Measures for Prevention of Food Borne Diseases Microbial Testing of Foods Conventional MethodsRapid Detection of Emerging High Risk Pathogens in Foods

Loss of the Otx2-Binding Site in the Nanog Promoter Affects the Integrity of Embryonic Stem Cell Subtypes and Specification of Inner Cell Mass-Derived Epiblast

• Otx2 binds to the promoter/enhancer region of Oct4, Sox2, and Nanog in ESCs and EpiSCs • Otx2 binding to the Nanog promoter helps maintain the integrity of ESC compartments • Loss of this Otx2-binding site induces primed-like features in ESCs • Otx2 regulation of Nanog contributes to ICM differentiation of the epiblast Mouse embryonic stem cells (ESCs) and the inner cell mass (ICM)-derived epiblast exhibit naive pluripotency. ESC-derived epiblast stem cells (EpiSCs) and the postimplantation epiblast exhibit primed pluripotency. Although core pluripotency factors are well-characterized, additional regulators, including Otx2, recently have been shown to function during the transition from naive to primed pluripotency. Here we uncover a role for Otx2 in the control of the naive pluripotent state. We analyzed Otx2-binding activity in ESCs and EpiSCs and identified Nanog, Oct4, and Sox2 as direct targets. To unravel the Otx2 transcriptional network, we targeted the strongest Otx2-binding site in the Nanog promoter, finding that this site modulates the size of specific ESC-subtype compartments in cultured cells and promotes Nanog expression in vivo, predisposing ICM differentiation to epiblast. Otx2-mediated Nanog regulation thus contributes to the integrity of the ESC state and cell lineage specification in preimplantation development.

Partito questa mattina il sistema di dissuasione con le telecamere

Sosta. De Corato: “Partito questa mattina il sistema di dissuasione con le telecamere per le auto in doppia fila sui principali assi stradali”

Bene street control, ingorghi a Milano per doppia fila e non per cantieri

Sosta: Auto in doppia fila addio...

Domani parte il sistema di dissuasione con le telecamere per le auto in doppia fila sui principali assi stradali

Auto in doppia fila: “Street Control“ il nuovo software per le “multe a strascico“